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Label-Free and Immobilization-Free Immunoassay

[+] Author Affiliations
Island Pinnick, Kieseok Oh, Chen-Ling Chang, Jae-Hyun Chung

University of Washington, Seattle, WA

Kyong-Hoon Lee

NanoFacture, Inc., Ann Arbor, MI

Paper No. IMECE2007-42370, pp. 37-40; 4 pages
  • ASME 2007 International Mechanical Engineering Congress and Exposition
  • Volume 2: Biomedical and Biotechnology Engineering
  • Seattle, Washington, USA, November 11–15, 2007
  • Conference Sponsors: ASME
  • ISBN: 0-7918-4296-7 | eISBN: 0-7918-3812-9
  • Copyright © 2007 by ASME


This paper presents an immunoassay capable of detecting an antigen without labeling or immobilization. By measuring a change in fluid resistance, the immunoassay successfully differentiates a positive control from a negative control. The same device can also act as a particle counter due to its high sensitivity. It is capable of detecting differences in concentrations as low as 104 particles per milliliter. An analytical model is developed to analyze the measured signal.

Copyright © 2007 by ASME



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