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Intracellular Ice Formation in Cell Suspensions Measured Using a Cryomicroscope and a Calorimeter

[+] Author Affiliations
Tathagata Acharya, Ram V. Devireddy

Louisiana State University, Baton Rouge, LA

Paper No. IMECE2007-41308, pp. 23-27; 5 pages
doi:10.1115/IMECE2007-41308
From:
  • ASME 2007 International Mechanical Engineering Congress and Exposition
  • Volume 2: Biomedical and Biotechnology Engineering
  • Seattle, Washington, USA, November 11–15, 2007
  • Conference Sponsors: ASME
  • ISBN: 0-7918-4296-7 | eISBN: 0-7918-3812-9
  • Copyright © 2007 by ASME

abstract

Injury to biological cells during the freezing-thawing process of a cryopreservation protocol is related to the thermodynamic state of the intracellular water. The two primary biophysical phenomena are water transport and intracellular ice formation (IIF). Unfortunately, there is no technique currently available to measure IIF in the cells of opaque tissue sections. In this proceeding we report the use of a calorimeter to assess IIF in two different cell suspensions, adult stem cells and pacific oyster embryos. The close agreement between the IIF data obtained using the calorimetric data with corresponding data obtained using a well-established cryomicroscopy technique validated the calorimetric method. Since, the calorimetric measurements are independent of shape and size, it is ideally suited to measure IIF in opaque tissue sections; the focus of future studies.

Copyright © 2007 by ASME
Topics: Ice

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