0

Full Content is available to subscribers

Subscribe/Learn More  >

Fabrication of 3D Cell Supporting Structures With Multi-Materials Using the Bio-Printer

[+] Author Affiliations
Yuichi Nishiyama, Makoto Nakamura, Chizuka Henmi, Kumiko Yamaguchi, Shuichi Mochizuki, Hidemoto Nakagawa, Koki Takiura

Kanagawa Academy of Science and Technology, Kawasaki, Kanagawa, Japan

Paper No. MSEC2007-31064, pp. 97-102; 6 pages
doi:10.1115/MSEC2007-31064
From:
  • ASME 2007 International Manufacturing Science and Engineering Conference
  • ASME 2007 International Manufacturing Science and Engineering Conference
  • Atlanta, Georgia, USA, October 15–18, 2007
  • Conference Sponsors: Manufacturing Division
  • ISBN: 0-7918-4290-8 | eISBN: 0-7918-3809-9
  • Copyright © 2007 by ASME

abstract

We produced three-dimensional (3-D) cell supporting structures for use in engineering regenerative living tissue. The structures were formed by alginate gel, a type of hydro-gel, and our originally developed printer, termed a 3-D bio-printer. A droplet ejected from an inkjet printer nozzle has the same size as several cells. Thus, we considered that such a printer nozzle would be able to eject cultured living cells, along with growth factor, protein, and other materials. If a 3-D gel structure could be formed with such cells, and the materials and cells self-assembled, a variety of living tissues could be obtained. In this report, our 3-D bio-printer and the method of fabrication of multiple material gel structures is presented. Our 3-D bio-printer has a printing mechanism that operates in 3 directions with a positioning resolution of 0.5 micrometers, which is adequate for precise positioning of the ejected cells. Further, a piezoelectric inkjet nozzle head that does not became heated during operation is used and cells can be ejected without heating. The head has 4 nozzles and is able to eject 4 different kinds of materials simultaneously. We used a sodium alginate solution and ejected it from the inkjet nozzle into a calcium chloride solution as a substrate, thus alginate gel beads were obtained. Several types of gel structures could be constructed by distributing the beads precisely and the resolution of the gel structures was as small as the size of an individual bead, about 10–60 μm in diameter. By continuous operation of the inkjet nozzle, gel lines were able to be formed. The substrate was a 10% calcium chloride solution on a slide glass and the ejected-droplets contained a 0.8% sodium alginate solution. In addition, gel sheets were formed by parallel gel lines. In that case, a 10% calcium chloride solution was also used as the substrate. It is possible that such 3-D gel sheet structures could be constructed by lamination in a high viscosity substrate. We also formed gel rings, which were stacked and allowed to sink into the substrate, thus obtaining gel tubes. By utilizing gel tubes with inner and outer walls formed using different types of gels with vascular endothelial cells and smooth muscle cells, blood vessel structures could be fabricated with the present system.

Copyright © 2007 by ASME
Topics: Manufacturing

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In