0

Full Content is available to subscribers

Subscribe/Learn More  >

Study of Vascular Endothelium Damage Due to Freezing

[+] Author Affiliations
Aili Zhang, Lisa X. Xu, George A. Sandison

Purdue University, West Lafayette, IN

Paper No. IMECE2003-41967, pp. 417-420; 4 pages
doi:10.1115/IMECE2003-41967
From:
  • ASME 2003 International Mechanical Engineering Congress and Exposition
  • Heat Transfer, Volume 4
  • Washington, DC, USA, November 15–21, 2003
  • Conference Sponsors: Heat Transfer Division
  • ISBN: 0-7918-3718-1 | eISBN: 0-7918-4663-6, 0-7918-4664-4, 0-7918-4665-2
  • Copyright © 2003 by ASME

abstract

Microvascular injury is recognized as a major tissue damage mechanism of ablative cryosurgery. Details of this injury mechanism are not completely understood. But it is known that extracellular ice propagating through the vascular region leads to endothelial cell dehydration, which may cause their detachment from each other and eventually from the vessel wall. Soon after post-thaw reperfusion vessel leakage is evident and thrombi form in the vessels leading to vascular stasis and consequently tissue ischemia. To better understand the mechanical principles underlying this tissue injury mechanism, we have modeled water transport phenomena that arise during the freezing of the vasculature. Endothelial cells were modeled as an independent but connected array of ellipsoidal balloons whose size varies according to the osmotic pressure experienced during the freezing process. An assumption of minimum surface area permeable to water was made as the mechanism governing the cell shape change. Variations in intercellular gap dimensions under different freezing protocols were obtained numerically. The results were then compared to the observed behavior of cultured endothelial cells undergoing the same freezing protocol on a cryostage. The simulation and experimental results are in good agreement.

Copyright © 2003 by ASME
Topics: Freezing

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In