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PhotoMEA: A New Step Towards Total Optical Analysis of In Vitro Neuronal Networks

[+] Author Affiliations
Diego Ghezzi, Alessandra Pedrocchi, Sara Mantero, Giancarlo Ferrigno

Politecnico di Milano, Milano, Italy

Andrea Menegon

San Raffaele Scientific Institute, Milano, Italy

Flavia Valtorta

San Raffaele “Vita-Salute” University, Milano, Italy

Paper No. ESDA2006-95218, pp. 441-449; 9 pages
doi:10.1115/ESDA2006-95218
From:
  • ASME 8th Biennial Conference on Engineering Systems Design and Analysis
  • Volume 2: Automotive Systems, Bioengineering and Biomedical Technology, Fluids Engineering, Maintenance Engineering and Non-Destructive Evaluation, and Nanotechnology
  • Torino, Italy, July 4–7, 2006
  • ISBN: 0-7918-4249-5 | eISBN: 0-7918-3779-3
  • Copyright © 2006 by ASME

abstract

Light stimulation of neurons is a promising approach for investigating the molecular mechanisms at the basis of neuronal physiology and plasticity. In particular, flash photolysis of caged compounds offers the unique advantage of allowing to quickly change the concentration of either intracellular or extracellular bioactive molecules, such as neurotransmitters or second messengers, for the stimulation or modulation of neuronal activity. In this field of research, we describe a simple laser-based set-up for the local activation of caged compounds. The coupling of a UV laser diode to a small-core optical fibre allows to reduce the uncaging area and to quickly change the stimulation point. The actual localisation of the light stimulation is determined using a caged fluorescent compound (dextran, DMNB-caged fluorescein). The efficiency of our set up for neuronal stimulation is tested with a caged neurotransmitter (MNI-caged-L-glutamate). Activation of caged glutamate evokes neuronal responses that are recorded using a MicroElectrode Array system and/or following the variations in the concentrations of the Cai 2+ . This work shows that our laser-based set-up is a powerful tool for local activation of caged compound allowing a unique opportunity to follow the effects of local neuronal pathways on neuronal network activity, for instance during pharmacological and toxicological treatments.

Copyright © 2006 by ASME
Topics: Networks

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