0

Full Content is available to subscribers

Subscribe/Learn More  >

The Effect of the Electrostatic Field on the Deflection of Microcantilever Biosensors

[+] Author Affiliations
Manuele Merlo, Fabio Negretto, Franco Maria Montevecchi

Politecnico di Torino, Torino, Italy

Paper No. ESDA2006-95155, pp. 415-422; 8 pages
doi:10.1115/ESDA2006-95155
From:
  • ASME 8th Biennial Conference on Engineering Systems Design and Analysis
  • Volume 2: Automotive Systems, Bioengineering and Biomedical Technology, Fluids Engineering, Maintenance Engineering and Non-Destructive Evaluation, and Nanotechnology
  • Torino, Italy, July 4–7, 2006
  • ISBN: 0-7918-4249-5 | eISBN: 0-7918-3779-3
  • Copyright © 2006 by ASME

abstract

Microcantilever biosensors offer the capability to detect specific molecular binding like complementary DNA fragment hybridization or specific antibody-antigen binding. The cantilever deflection, which can be optically detected, is caused by the adsorption of biological molecules like DNA fragments upon the microcantilever surface and the subsequent specific binding to the complementary species. The cantilever deflection is due to the surface stress induced by the free energy variation on the cantilever surface. Contributions to the free energy variation come from a number of interactions within the molecules, such as electrostatic interactions, biomolecule conformational entropy and internal energy variation, hydration forces. In the present work the effect of the electrostatic field within DNA biomolecules on the cantilever deflection is investigated. The electrostatic field within double strand DNA molecules is studied by means of a Finite Element (FE) analysis aimed at numerically solving the non linear Poisson Boltzmann equation (PBE) in the domain representing the biomolecule system. The electrostatic analysis has been coupled to a FE structural analysis in order to evaluate the influence of the electrostatic field on the cantilever deflection. The double strand DNA molecules are modelled as a periodic disposition of cylinders negatively charged at the surface. The hexagonal and square DNA molecule patterns were compared, and the Manning condensation hypothesis was discussed. The results are shown for different operating conditions and compared with experimental data from literature.

Copyright © 2006 by ASME

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In