0

Full Content is available to subscribers

Subscribe/Learn More  >

Insulator Based Dielectrophoresis: Effects of Bulk Medium Properties

[+] Author Affiliations
Blanca H. Lapizco-Encinas, Sandra Ozuna-Chacón, Marco Rito-Palomares, Esther Collado-Arredondo, Sergio O. Martínez-Chapa

Tecnológico de Monterrey, Monterrey, N.L., Mexico

Claudia Reyes-Betanzo

INAOE, Tonantzintla, Puebla, Mexico

Paper No. ICNMM2007-30049, pp. 177-183; 7 pages
doi:10.1115/ICNMM2007-30049
From:
  • ASME 2007 5th International Conference on Nanochannels, Microchannels, and Minichannels
  • ASME 5th International Conference on Nanochannels, Microchannels, and Minichannels
  • Puebla, Mexico, June 18–20, 2007
  • Conference Sponsors: Nanotechnology Institute
  • ISBN: 0-7918-4272-X | eISBN: 0-7918-3800-5
  • Copyright © 2007 by ASME

abstract

Dielectrophoresis (DEP) is the motion of a particle due to polarization effects in nonuniform electric fields. DEP is an electrokinetic transport mechanism that can be used to concentrate and separate particles. DEP is a nondestructive technique, with a great potential for the separation and concentration of bioparticles. Traditionally, DEP has been carried out employing arrays of microelectrodes to generate nonuniform electric fields. This approach is expensive due to the elevated cost of microelectrode fabrication, which makes high throughput systems economically unfeasible. An alternative is the technique called insulator-based DEP (iDEP). In this technique, nonuniform electric fields are created with an array of insulating structures, instead of electrodes. Insulating materials such as plastics have excellent malleability and can be mass replicated, providing for high-throughput and large-volume devices. The present study investigated the effect of bulk medium properties on the dielectrophoretic behavior of microparticles under iDEP, employing microdevices made from glass. Each microdevice contained several microchannels, and each microchannel contained an array of insulating cylindrical posts. Electric field was applied across the post array, creating regions of higher and lower electric field intensity. Prior to each experimental session, the microchannel was filled with a buffer solution of a known pH and conductivity. A sample of the microparticles was injected into the microchannel and an electric field was applied. The dielectrophoretic response of the particles was recorded in the form of videos and pictures. A parametric study was carried out by varying the pH and conductivity of the bulk medium, as well as the magnitude of the applied electric field, in order to study how each one of these parameters affects the dielectrophoretic response of the microparticles. It is anticipated that the results from this research project will provide with guidelines for the design and operation of insulator-based DEP devices for the concentration and separation of bioparticles.

Copyright © 2007 by ASME

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In