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Two-Photon Polymerization Technique for Bio-Applications

[+] Author Affiliations
Aleksandr V. Ovsianikov, Boris N. Chichkov

Laser Zentrum Hannover e.V.

Paper No. IMECE2005-79873, pp. 105-108; 4 pages
doi:10.1115/IMECE2005-79873
From:
  • ASME 2005 International Mechanical Engineering Congress and Exposition
  • Manufacturing Engineering and Materials Handling, Parts A and B
  • Orlando, Florida, USA, November 5 – 11, 2005
  • Conference Sponsors: Manufacturing Engineering Division and Materials Handling Division
  • ISBN: 0-7918-4223-1 | eISBN: 0-7918-3769-6
  • Copyright © 2005 by ASME

abstract

We will report on the recently developed two-photon polymerization (2PP) technique for biological applications. 2PP of photosensitive inorganic-organic hybrid polymers (ORMOCERs) is applied for the fabrication of 3D scaffolds, micro-and nanostructures. ORMOCERs have negative resist behaviour and can be used as storage-stable, liquid photopolymerizable resins. When Ti:sapphire femtosecond laser pulses are tightly focused into the volume of this resin (which is transparent in the infrared) they initiate 2PP process transferring liquid into the solid state. This allows the fabrication of any computer-generated 3D structure by direct laser “recording” into the volume of this material. ORMOCERs demonstrate excellent cell adherence and cell growth rate comparable to that of Extracellular matrix. With 2PP technique there are no limits in complexity of the 3D scaffold structures which can be fabricated with the resolution of 100 nm.

Copyright © 2005 by ASME

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