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Statistical Particle Tracking Velocimetry Using Molecular and Quantum Dot Tracer Particles

[+] Author Affiliations
Jeffrey S. Guasto, Peter Huang, Kenneth S. Breuer

Brown University

Paper No. IMECE2005-80051, pp. 235-244; 10 pages
doi:10.1115/IMECE2005-80051
From:
  • ASME 2005 International Mechanical Engineering Congress and Exposition
  • Fluids Engineering
  • Orlando, Florida, USA, November 5 – 11, 2005
  • Conference Sponsors: Fluids Engineering Division
  • ISBN: 0-7918-4219-3 | eISBN: 0-7918-3769-6
  • Copyright © 2005 by ASME

abstract

We present the theory and experimental validation of a particle tracking velocimetry algorithm developed for application with nanometer-sized tracer particles such as fluorescent molecules and quantum dots (QDs). Traditional algorithms are challenged by extremely small tracers due to difficulties in determining the particle center, shot noise, high drop-in/drop-out and, in the case of quantum dots, fluorescence intermittency (blinking). The algorithms presented here determine real velocity distributions from measured particle displacement distributions by statistically removing randomly distributed tracking events. The theory was verified through tracking experiments using 54 nm flourescent dextran molecules and 6 nm QDs.

Copyright © 2005 by ASME

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