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Automated Sample Preparation System for Rapid Biological Threat Detection

[+] Author Affiliations
Jeanne C. Stachowiak, Erin E. Shugard, Pamela Caton, Bruce P. Mosier, Ron Renzi, Rafael V. Davalos, Gregory J. McGraw, Blake A. Simmons, Victoria A. Vandernoot, Brent A. Haroldsen

Sandia National Laboratories

Paper No. IMECE2005-80945, pp. 29-30; 2 pages
  • ASME 2005 International Mechanical Engineering Congress and Exposition
  • Advances in Bioengineering
  • Orlando, Florida, USA, November 5 – 11, 2005
  • Conference Sponsors: Bioengineering Division
  • ISBN: 0-7918-4213-4 | eISBN: 0-7918-3769-6
  • Copyright © 2005 by ASME


Rapid, automated sample preparation of bacterial cells and spores is required for threat analysis by remotely deployed chemical and biological warning systems. Sandia is designing, building, and testing an automated front-end sample preparation system based on miniature and microfluidic components, with the goal of concentrating bacterial species collected from the air, harvesting and solubilizing proteins from them, and delivering them to Sandia’s MicroChemLab capillary gel electrophoresis system1,2 for analysis (Fig. 1). Miniature, motorized valves and pumps control flow between system components connected by fused silica capillaries (Fig. 4). Sample processing modules include concentration by dielectrophoresis in an array of insulating posts or by mechanical filtration; heat-activated chemical lysis; mechanical filtration; removal of chemical lysis agents by size exclusion chromatography (SEC); and in-capillary fluorescent labeling.

Copyright © 2005 by ASME



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