Full Content is available to subscribers

Subscribe/Learn More  >

An Integrated Microfluidic Dielectrophoretic (DEP) Cell Fractionation System

[+] Author Affiliations
Youlan Li, Colin Dalton, Humud Said, Karan V. I. S. Kaler

University of Calgary, Calgary, AB, Canada

Paper No. ICMM2005-75062, pp. 403-410; 8 pages
  • ASME 3rd International Conference on Microchannels and Minichannels
  • ASME 3rd International Conference on Microchannels and Minichannels, Part B cont’d
  • Toronto, Ontario, Canada, June 13–15, 2005
  • Conference Sponsors: Nanotechnology Institute
  • ISBN: 0-7918-4185-5 | eISBN: 0-7918-3758-0
  • Copyright © 2005 by ASME


In this article we report on a planar miniaturized dielectrophoretic (DEP) microfluidic device developed for the purpose of continuous fractionation and purification of sample suspensions of microscopic particles or biological cells, employing specially shaped nonuniform (isomotive) electric fields. The device integrates three fully functional and distinct sub-units consisting of 1) sheath and sample injection ports, arranged to achieve hydrodynamic focusing of the cell stream; 2) the DEP fractionation region and 3) two sample collection ports. In the DEP fractionation region, the magnitude of the field induced DEP force acting on the particle is essentially constant and independent of the particle’s position and furthermore only dependent on the intrinsic polarization response of the particle, for identical sized particles. The operation and performance in terms of sample throughput, separation efficiency and repeatability of the device was evaluated using test microscopic sized dielectric particles and biological particles, including cancerous cell lines.

Copyright © 2005 by ASME
Topics: Microfluidics



Interactive Graphics


Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In