Full Content is available to subscribers

Subscribe/Learn More  >

DNA Purification of Biothreat Agents Using a Micropillar Chip

[+] Author Affiliations
Kevin D. Ness, Raymond P. Mariella, Jr.

Lawrence Livermore National Laboratory, Livermore, CA

Gary W. Long, Phillip Belgrader

Tetracore, Gaithersburg, MD

Kenneth E. Goodson

Stanford University, Stanford, CA

Paper No. ICMM2005-75246, pp. 769-778; 10 pages
  • ASME 3rd International Conference on Microchannels and Minichannels
  • ASME 3rd International Conference on Microchannels and Minichannels, Parts A and B
  • Toronto, Ontario, Canada, June 13–15, 2005
  • Conference Sponsors: Nanotechnology Institute
  • ISBN: 0-7918-4185-5 | eISBN: 0-7918-3758-0
  • Copyright © 2005 by ASME


Processing large volume liquid samples for PCR-based infectious agent testing is ubiquitous among a wide variety of environmental samples. A number of different extraction protocols and devices are available to purify the nucleic acids from these complex samples. However, most of these approaches are optimized for specific kinds of samples and typically involve benchtop equipment and highly skilled personnel. Among the most common purification techniques are those that utilize a combination of chaotropic agents and random surfaces of glass (packed beds of micro-beads, fibers, particles, etc.) in a simple disposable plastic device. As an alternative surface, we have exploited the glass-surface properties of oxidized single crystal silicon in high-surface-area microstructures for silicon oxide-mediated nucleic acid extraction, purification, and concentration from large volume samples. One particular microstructure, a silicon pillar chip, provides highly-ordered and controlled surface interactions. The high aspect ratio (∼40) of the pillars provides an immense surface area within a relatively small volume, thus allowing for the capability to concentrate nucleic acids by several orders of magnitude. Here, we report on the effectiveness of this microfluidic chip in processing Francisella DNA in wastewater. The flow-through properties of the microfluidic chip enabled the entire procedure to be automated by embedding the chip in a reusable microfluidic cassette.

Copyright © 2005 by ASME
Topics: DNA



Interactive Graphics


Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In