0

Full Content is available to subscribers

Subscribe/Learn More  >

Using a Laminar Flow System to Explain Shear-Enhanced Bacterial Adhesion

[+] Author Affiliations
Wendy E. Thomas

University of Washington, Seattle, WA

Paper No. ICMM2005-75217, pp. 751-759; 9 pages
doi:10.1115/ICMM2005-75217
From:
  • ASME 3rd International Conference on Microchannels and Minichannels
  • ASME 3rd International Conference on Microchannels and Minichannels, Parts A and B
  • Toronto, Ontario, Canada, June 13–15, 2005
  • Conference Sponsors: Nanotechnology Institute
  • ISBN: 0-7918-4185-5 | eISBN: 0-7918-3758-0
  • Copyright © 2005 by ASME

abstract

Microfluidic parallel plate flow chambers provide a method for evaluating the role of molecular mechanics in the dynamic adhesion of cells or bacteria. We use optical microscopy for real-time monitoring of adherent cells in laminar flow conditions to study a counter-intuitive observation. Escherichia coli, our most common intestinal bacteria, require shear stress to bind to surfaces coated with mannose, a carbohydrate expressed on many host cells. In fact, even cells that are already bound will detach if the flow is turned down. Because these flow chambers involve bacteria binding to surfaces similar to biomaterial or tissue surfaces in our body, these experiments shed light on bacterial adhesion in vivo. However, flow chamber experiments can be carefully controlled so that they can also be used to determine the mechanism behind this unusual behavior. We show that the effect of shear stress on transport of bacteria to the surface cannot explain these results. Rather, increased shear stress causes bacteria to roll instead of detach and to switch to a stationary mode of adhesion.

Copyright © 2005 by ASME

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In