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Detection of Directivity of Fluorescence From a Mixed Specimen Which Consists of Micro Particles Attached Different Fluorescent Substances Using a Light Waveguide Incorporated Optical TAS

[+] Author Affiliations
Toshifumi Ohkubo, Nobuyuki Terada, Yoshikazu Yoshida

Toyo University, Kawagoe, Japan

Paper No. ISPS-MIPE2018-8503, pp. V001T05A001; 3 pages
doi:10.1115/ISPS-MIPE2018-8503
From:
  • ASME-JSME 2018 Joint International Conference on Information Storage and Processing Systems and Micromechatronics for Information and Precision Equipment
  • ASME-JSME 2018 Joint International Conference on Information Storage and Processing Systems and Micromechatronics for Information and Precision Equipment
  • San Francisco, California, USA, August 29–30, 2018
  • Conference Sponsors: Information Storage and Processing Systems Division
  • ISBN: 978-0-7918-5193-7
  • Copyright © 2018 by ASME

abstract

A resin-based optical total analysis system (O-TAS) which consists both of microfluidic channels and light waveguides [1] is thought to be one of the most promising components in developing a “ubiquitous human healthcare system” in the near future. Along with this technology trend, we have already developed a transparent epoxy-resin-based optical TAS chip which has a specially prepared light waveguide structure of radially arranged configuration at an intersection portion with a microfluidic channel, in order to detect directivity of fluorescence from fluorescent substance attached micro particles [2],[3]. Schematic diagram of the optical TAS is shown in Figure 1. In the latest research, utilizing an AC modulated laser source and time-series averaging function on detected signal waveforms, we could have successfully obtained directivities of fluorescence from 5-μm-diameter particles with higher signal to noise (S/N) ratio [3].

Copyright © 2018 by ASME

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